Harvard Medical School The Evolution Of Bacteria Mega-Plate Petri Dish

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Harvard Medical School The Evolution Of Bacteria Mega-Plate Petri Dish

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[Harvard Medical School The Evolution Of Bacteria Mega-Plate Petri Dish]

[Michael Baym (Narrator):] Source: LYBIO.net
So what we ended up building was basically a Petri dish, except that it’s 2 feet by 4 feet. And the way we set it up is that there are nine bands, and at the base of each of these bands, we put a normal Petri-dish-thick agar with different amounts of antibiotic.

On the outside there’s no antibiotic; just in from that there’s barely more than the E. coli can survive; inside of that, there’s 10 times as much; a 100 times; and then finally the middle band has a thousand times as much antibiotic. And then across the top of it we pour some thin agar that bacteria can move around in.

The background is black because there’s ink in it, and the bacteria appear as white.

First you see they spread in the area where there’s no antibiotic up until the point that can no longer survive. Then a mutant appears on the right. It’s resistant to the antibiotic. It spreads until it starts to compete with other mutants around it.

When these mutants hit the next boundary, they too have to pause and develop new mutations to make it into 10 times as much antibiotic. And then you see the different mutants repeat this at 100, and after about 11 days, they finally make it into 1000 times as much antibiotic as the wild-type can survive.

And so we can see by this process of accumulating successive mutations that bacteria which are normally sensitive to an antibiotic can evolve resistance to extremely high concentrations in a short period of time.

Harvard Medical School The Evolution Of Bacteria Mega-Plate Petri Dish

Harvard Medical School The Evolution Of Bacteria Mega-Plate Petri Dish

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HARVARD
MEDICAL SCHOOL

Research and video by Michael Baym, Tami Lieberman, Eric Kelsic, Remy Chait, Rotem Gross, Idan Yelin and Roy Kishony

Narrated by Michael Baym
Edited by Rick Groleau

Music courtesy of Extreme Music
“Under the Knife”
written by David Russell Fleming and Joerg Huettner

(C) 2016 The President and Fellows of Harvard College

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